Translation and Tethering (TnT) Plasmid

For Live Cell Imaging

At a Glance

Researchers at Colorado State University have developed fluorescence microscopy tools to image proteins in living cells that are difficult, if not impossible to see using standard techniques. These techniques can visualize the translation of DNA, and answer long-standing questions about how epigenetic factors contribute to gene misregulation in human disease. Currently  these plasmids have been utilized on two hard-to-see protein populations such as: (1) nascent proteins in the process of being translated (while still attached to RNA) and (2) post-translationally modified proteins, particularly modified chromatin and modified transcription machinery.

The “Translation and Tethering” TnT biosensor is a combination of the nascent chain tracking translation reporter and mRNA tethering reporters. The TnT biosensor available today is adaptable to any factor since tethering/recruitment is through a tethering cassette and not the endogenous RNA binding domain of a specific factor. TnT can be used to screen effects such as DNA translation as well as up and down regulation of proteins. Additionally, TnT can be used to study translation factors as well as effects of subcellular localization on translation by tethering TnT mRNA to a specific location.

Follow the link below to find more details about the product and purchase for laboratory use.

Overview

Translation and Tethering (TnT) Plasmid

TRANSLATION AND TETHERING (TNT) INFO AND PURCHASE

The TnT plasmid is a nucleic acid-based biosensor to measure the specific impacts of DNA translation control factors by microscopy.

Highlights:

  • Combination of the nascent chain tracking translation reporter and mRNA tethering reporters
  • Adaptable to any factor since tethering/recruitment is through a tethering cassette
  • Useful for screening DNA translation as well as up/down regulation of proteins and gene expression
  • Study translation factors as well as effects of subcellular localizations
  • Single cell resolution of translation factors and methods
Last Updated: January 2024
High-tech plasmid microscopy equipment
Opportunity

Available for Licensing through Kerafast

Inventors

Timothy Stasevich
Charlotte Cialek

Reference Number
2021-087
Licensing Manager

Steve Foster
Steve.Foster@colostate.edu
970-491-7100