Researchers at Colorado State University have developed a series of DNA plasmids and protocols for generating and purifying recombinant antibodies from HEY293 cells. This protocol can produce high-yield recombinant monoclonal antibodies at a relatively low cost.
Follow each link to find more details about each product.
pDL026_HA-rb_IgG_LC_15F11 Info/Purchase
HA-tag light chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit light chain constant regions + exogenous N-term signal peptide. For co-expression with pDL025
pDL025_HA-rb_IgG_HC_15F11 Info/Purchase
HA-tag heavy chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit heavy chain constant regions + exogenous N-term signal peptide. For co-expression with pDL026.
pDL024_Tub-rb_IgG_LC Info/Purchase
α-Tubulin light chain variable region + rabbit light chain constant region + exogenous N-term signal peptide. For co-expression with pDL023 in HEK293 cells.
pDL023_Tub-rb_IgG_HC Info/Purchase
α-Tubulin heavy chain variable region + rabbit heavy chain constant regions + exogenous N-term signal peptide. For co-expression with pDL024 in HEK293 cells.
pDL002_Hec1-ms_IgG_LC Info/Purchase
Hec1 light chain variable and constant region (mouse) + exogenous N-term signal peptide. For co-expression with pDL001 in HEK293 cells.
pDL001_Hec1-ms_IgG_HC Info/Purchase
Hec1 heavy chain variable and constant region (mouse) + exogenous N-term signal peptide. For co-expression with pDL002 in HEK293 cells.
Available for Purchase at Addgene. Individual products linked.
Jennifer DeLuca
Steve Foster
Steve.Foster@colostate.edu
970-491-7100