Researchers at Colorado State University have developed novel cell lines which have been modified to not allow DNA repair. These cells can be utilized in experiments to study drugs targeting specific gene mutant cells such as BRCA1 mutated cells. These cells lines, based on the well characterized Chinese hamster CHO cells and V79 cells, grow rapidly and can be effectively used to screen drugs in development.
There is a large gap in the panel of Chinese Hamster mutant cell lines, which are model mammalian cell lines. These cell lines have been engineered to have mutations in ATM, ATR, or BRCA1.
These cell lines have been engineered to have mutations in ATM, ATR, BRCA1, which allow of precise analysis in radiobiology and cancer biology. Using CRISPR/Cas9, 5 cell lines were established, 2 which are knockouts of ATM and two mutants of ATR, and 1 mutant of BRCA1.
• Study DNA damage response for genes such as ATM, ATR, or BRCA1
• Good models for impaired ATR function and Seckel syndrome
• Easily research specific gene mutations
• Stable chromosome number
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Takamitsu Kato
Junko Maeda
Steve Foster
Steve.Foster@colostate.edu
970-491-7100