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Next-Generation Auxin Herbicide Resistance for Crops

Novel resistance trait and molecular marker for dicots

At a Glance

Researchers at Colorado State University have developed a new herbicide-resistance trait for dicot crops such as cotton, soybean, and tomato. The trait enables tolerance to widely used auxin herbicides, including dicamba and 2,4-D. The team also created a DNA marker to quickly identify resistant plants. These tools support faster and more precise breeding of herbicide-tolerant crops.

Background

Auxin herbicides remain a key tool for controlling broadleaf weeds, including those resistant to other chemistries like glyphosate. However, many dicot crops are sensitive to these herbicides, which limits their use in production systems. A naturally occurring mutation first identified in Bassia scoparia revealed a new mechanism of resistance that does not rely on herbicide breakdown. Recent research shows this mutation alters auxin signaling at the receptor level, offering a novel and transferable trait. This approach could expand weed-control options while helping maintain crop yield and reduce resistance pressure.

Overview

The technology is based on a mutation in the AUX/IAA16 gene that changes how plants respond to auxin herbicides. An insertion disrupts normal RNA splicing, producing a modified protein that weakens interaction with the receptor complex. Because auxin herbicides rely on this interaction to trigger plant death, the reduced binding lowers herbicide sensitivity while preserving normal growth.

New findings further confirm that this mutation reduces degradation of AUX/IAA16 in the presence of auxins, stabilizing the protein and limiting downstream signaling. In Arabidopsis thaliana, engineered lines showed strong tolerance to dicamba and 2,4-D, with minimal impact on root growth under treatment. A linked molecular marker enables rapid screening, and the trait can be deployed through gene editing, transgenic approaches, or mutagenesis. The function of this mutation has also been demonstrated in tobacco and soybean, data available upon request.

Figure 1. Dose-response curves show higher tolerance to dicamba and 2,4-D in resistant versus sensitive plant lines, with resistant plants maintaining root growth at herbicide concentrations that inhibit wild-type plants.

Benefits

  • Enables tolerance to auxin herbicides such as dicamba and 2,4-D
  • Expands control of glyphosate-resistant and other hard-to-manage weeds
  • Introduces a novel mechanism acting at the hormone signaling level
  • Includes a molecular marker for faster breeding and selection
  • Maintains normal plant growth and development under non-treated conditions
  • Applicable across multiple dicot crops (cotton, soybean, tomato, sunflower)
  • Flexible deployment via gene editing, mutagenesis, or transgenic methods
  • Supports more sustainable weed management and yield stability

Applications

  • Development of herbicide-tolerant crop varieties
  • Seed company breeding programs using diagnostic markers
  • Integrated weed-management systems in row crops
  • Trait stacking with other herbicide resistance technologies
  • Research on auxin signaling and plant hormone pathways

Publications

Montgomery, J. S., et al. (2025) “A transposable element insertion in AUX/IAA16 disrupts splicing and causes auxin resistance in Bassia scoparia.” The Plant Journal. https://doi.org/10.1111/tpj.70339

T. Gaines, et al (2020) “Mechanisms of evolved herbicide resistance/” Journal of Biological Chemistry. https://doi.org/10.1074/jbc.REV120.013572

 

Last Updated: April 2026
Even rows of healthy crops in a field, showing modern farming with auxin herbicide-resistant plants.
Opportunity

Available for Exclusive Licensing
TRL: 3

IP Status

PCT/US2025/012557

Inventors

Todd Gaines
Jacob Montgomery
Neeta Soni-Castillo
Eric Patterson
Eric Westra
Sarah Morran

Reference Number
2023-044
Licensing Manager

Jessy McGowan
Jessy.McGowan@colostate.edu
970-491-7100

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