HCT116 Cell Lines to Study Deubiquitation

At a Glance

Researchers at Colorado State University have developed series of cell lines we created to study the function of the deubiquitinase UCH37 (also known as UCHL5). These 4 cell lines that are isogenic except for the form of UCH37 and are readily usable in drug screening assays.

Overview

Starting with parental HCT116 cells, CRISPR technology was used to knockout both copies of endogenous UCH37 gene to create UCH37KO. Subsequently, different versions of GFP-Flag-tagged UCH37was introduced into UCH37KO cells to query the function of UCH37. These include: wild type (WT), catalytically-inactive (C88A), and ubiquitin binding deficient (EWI).

Benefits

Probe the function of branched polyUb chains
Cell lines available for in vitro studies

Applications

Proteasome degradation

Publications

Yao, T. et al. (2021) Branched ubiquitin chain binding and deubiquitination by UCH37 facilitate proteasome clearance of stress-induced inclusions. eLife https://elifesciences.org/articles/72798

Last Updated: October 2024

Opportunity

Available for Purchase biological reagent only. Cell lines are available through Applied Biological Materials.

Inventors

Tingting Yao

Reference Number

2024-087

Licensing Manager

Steve Foster
Steve.foster@colostate.edu
970-491-7100