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AncestraVax™

At a Glance

Researchers at Colorado State University have developed a universal vaccine approach to protect against multiple dangerous tick-borne viruses from the flavivirus family. The vaccine uses “ancestral antigens” that provide immunity against both known and emerging viruses. This strategy targets a wide range of pathogens with a single formulation, offering a major step forward over traditional vaccines. It represents a promising tool for global health defense against diseases that currently lack effective prevention.

Background

Tick-borne flaviviruses (TBFs) cause severe neurological disease and are responsible for significant illness and death worldwide. Existing vaccines are either unavailable, narrowly targeted, or require multiple booster doses, leaving populations vulnerable. The CSU technology addresses this gap by introducing a broadly protective vaccine platform that works across multiple virus strains. This innovation is particularly important as climate change and expanding tick habitats are increasing the geographic spread of these diseases.

Overview

This technology focuses on creating a cross-protective vaccine against tick-borne flaviviruses by reconstructing the common ancestors of viral proteins. Using maximum likelihood modeling, researchers designed ancestral envelope (E) and non-structural (NS1) proteins that represent the evolutionary “roots” of multiple related viruses. By expressing these proteins in established vaccine platforms (the technology stimulates antibody-mediated responses).

Preclinical data show that mice vaccinated with the Sindbis constructs develop a strong neutralizing antibodies and survive otherwise lethal virus challenges. Importantly, this strategy provides immunity not just to specific strains like tick-borne encephalitis virus (TBEV) or Powassan virus (POWV) but also offers broad protection across the tick-borne flaviviruses. Unlike current strain-specific vaccines, the CSU approach anticipates future viral threats of related tick-borne flaviviruses by targeting their common evolutionary origins.

Figure 1: Female C57/BL6 mice (n=8) vaccinated with 105 PFU of AncestraVax and boosted at day 21 showed increased survival to lethal Powassan virus (POWV) challenge.
Figure 2: Sera harvested from AncestraVax vaccinated mice (n=4) generate strongly-neutralizing antibodies that reduce infectivity of Powassan virus (POWV)
Figure 3: Sera harvested from AncestraVax vaccinated mice (n=4) generate strongly neutralizing antibodies that reduce infectivity of the related Langat virus (LGTV) (~78% identity of the Envelope protein).

Benefits

  • Broad-spectrum protection against multiple tick-borne flaviviruses.
  • Designed to work against both known and emerging viral threats.
  • Stimulates humoral (antibody) immunity.
  • Potentially reduces or eliminates the need for multiple booster shots.
  • Adaptable to multiple vaccine delivery systems (attenuated viruses, replicons).
  • Strong preclinical evidence of survival benefit in lethal infection models.

Applications

  • Human vaccines for global populations at risk of tick-borne encephalitis, Powassan virus, and related diseases.
  • Military and first-responder protection against biothreat-level pathogens.
  • Veterinary vaccines for animals exposed to tick-borne viruses.
  • Public health preparedness tools for regions facing emerging tick-borne virus outbreaks.
  • Platform adaptable to other virus families using the same ancestral antigen strategy.
Last Updated: October 2025
Close-up of a tick on human skin.
Opportunity

Available for Exclusive Licensing, Collaboration or Funding
TRL: 5

IP Status

US Provisional Patent

Inventors

Greg Ebel
Brian Geiss
Chasity Trammell

Reference Number
2025-093
Licensing Manager

Steve Foster
Steve.Foster@colostate.edu
970-491-7100

Contact Us About this Technology
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