Sindbis Virus Replicons and Plasmids: Used to Study a Wide Range of Cellular Processes

Replicon Producing Plasmids

pBG60 Recombinant Sindbis Replicon Expression Plasmid—Gateway Compatible

PBG60 INFO/PURCHASE

Recombinant plasmid pBG60 containing Sindbis virus (SINV) that allows for fast gene of interest addition with Gateway recombination.

Highlights:

• Contains a Gateway recombination cassette for rapid addition of genes of interest into the replicon 3’ subgenomic promoter
• Expresses Blasticidin-S-deaminase (BSD) protein for selection
• Can be used in poorly or non transfectable cells when co-transfected with the packaging plasmid pBG256

pBG68 Recombinant Sindbis Replicon Expression Plasmid

PBG68 Info/Purchase

Recombinant plasmid pBG68 produces Sindbis virus (SINV) replicons in cells that allows for fast and easy expression of your gene of interest.

Highlights:

• Can be transfected into mammalian cells to produce replicating but non-infectious Sindbis virus genomes
• Expresses Blasticidin-S-deaminase (BSD) protein for selection
• Can be used in poorly transfectable cells when co-transfected with the packaging plasmid pBG256

pBG78 Recombinant Sindbis Replicon Plasmid – GFP Expressing

PBG78 Info/Purchase

Recombinant plasmid pBG78 produces Sindbis virus (SINV) replicons in cells that can be easily visualized via GFP expression.

Highlights:

• Can be transfected into mammalian cells to produce replicating but non-infectious Sindbis virus genomes
• Expresses GFP for easy visualization of viral genome replication in live cells
• Expresses Blasticidin-S-deaminase (BSD) and Green Fluorescent Protein (GFP)
• Can be used in poorly transfectable cells when co-transfected with the packaging plasmid pBG256

pBG256 Recombinant Sindbis Virus Replicon Packaging Plasmid

PBG256 Info/Purchase

Recombinant packaging plasmid pBG256 lacking the viral nsP1-nsP4 replication genes and can be used to combine plasmids with pBG60, pBG68, pBG78.

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Infectious Virus Producing Plasmids

pBG167 Recombinant Sindbis Virus Expression Plasmid

PBG167 Info/Purchase

Recombinant plasmid pBG167 expressed infectious Sindbis Virus (SINV) and allows for fast and easy expression of your gene of interest.

Highlights:

• Plasmid can be transfected into mammalian cells to produce infectious Sindbis virus
• Unique XbaI site behind 3’ subgenomic promoter can be used to express genes of interest during infection

pBG210 Recombinant Sindbis Virus Expression Plasmid – Gateway Compatible

PBG210 Info/Purchase

Recombinant plasmid pBG210 containing Sindbis Virus (SINV) allows for fast gene of interest addition with Gateway recombination.

Highlights:

• Contains a Gateway recombination cassette to allow for efficient addition of genes of interest behind 3’ subgenomic promoter

pBG218 Recombinant Sindbis Virus-GFP Expression Plasmid

PBG218 Info/Purchase

Recombinant plasmid pBG218 expresses Sindbis virus (SINV) expressing GFP during viral replication for easy visualization.

Highlights:

• Plasmid can be transfected mammalian cells to produce infectious Sindbis virus
• Expresses Green Fluorescent Protein (GFP) for easy visualization of infection