Plasmids for Replacement of Nicotiana Plastid clpP1 Gene with Silene Orthologs

For Studying Neuronal Calcium Handling In Vivo

At a Glance

These plasmids are used for plastome modification via particle bombardment to replace the native clpP1 gene in Nicotiana with homologous copies from various Silene species. They can be used to study subcellular calcium handling in vivo, a key pathway in neuronal function and studies.

Overview

Below is a detailed description of plasmid.

Plasmids for replacement of Nicotiana plastid clpP1 gene with Silene orthologs

These plasmids enable the rapid acquisition of differential Ca2+ levels within the subcellular regions and organelles of individual neurites in vivo with high spatial resolution.

PBS-V4

PBS-V6

PBS-V7

PBS-V9

Benefits

  • Allows for studies of subcellular calcium dynamics in vivo
  • Neurons can be studied in native, intact circuitry
  • Resolution of calcium dynamics down to the individual dendritic spines and mitochondria

Applications

  • Live neuronal studies
  • Calcium handling regulation of neuronal function and plasticity, and consequent disfunction in disease models
Last Updated: April 2023
Imaging of a neuron
Inventors

Dan Sloane

Reference Number
2023-007
Licensing Manager

Steve Foster
Steve.Foster@colostate.edu
970-491-7100