HA Frankenbody Expression Plasmids

For Live Cell Imaging

At a Glance

Researchers at Colorado State University have developed fluorescence microscopy tools to image proteins in living cells that are difficult if not impossible to see using standard techniques. They are using these techniques to answer long-standing questions about how epigenetic factors contribute to gene misregulation in human disease. Currently they are focusing on two hard-to-see protein populations: (1) nascent proteins in the process of being translated (while still attached to RNA) and (2) post-translationally modified proteins, particularly modified chromatin and modified transcription machinery.

These HA Frankenbody Expression Plasmids genetically encode scFvs that selectively bind the HA epitope (YPYDVPDYA) with high affinity in living cells and organisms and can be used to quantify HA-tagged protein translation, localization, and dynamics. The plasmids enable, state-of-the-art, single-molecule experiments in living cells, which we demonstrate by tracking single HA-tagged histones in U2OS cells and single mRNA translation dynamics in both U2OS cells and neurons.

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Overview

HA Frankenbody Expression Plasmids

PNZ035, Pnz055 & pnz053 info/purchase

Plasmids encoding anti-HA single-chain variable fragments (scFv) fused with mEGFP, HaloTag, or mCherry under CMV promoter.

Highlights:

  • Encode mouse anti-HA- scFvs for H. sapiens (human) expression
  • Engineered scFv binding the linear HA epitope with high affinity and specificity in vivo
  • Light up in multiple colors HA-tagged nuclear, cytoplasmic, membrane, and mitochondrial proteins in diverse cell types
  • Precisely quantify the expression of HA-tagged proteins in live cells
  • Powerful tool for imaging protein dynamics in vivo
Last Updated: August 2023
Microscope
Inventors

Timothy Stasevich
Ning Zhao
Hiroshi Kimura
Yuko Sato

Reference Number
18-093
Licensing Manager

Steve Foster
Steve.Foster@colostate.edu
970-491-7100