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Inventor
Reference No: 2020-090
Licensing Manager
Steve Foster
Steve.Foster@colostate.edu
970-491-7100
At a Glance
Researchers at Colorado State University have developed several plasmids and purification protocols for the expression and purification of a soluble SARS-CoV-2 nucleocapsid protein for use in diagnostic assays, in response to the SARS-CoV-2/COVID-19 outbreak.
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SARS-CoV-2 Nucleocapsid Protein Expression Plasmid
pBG690 SARS-CoV-2 Nucleocapsid Protein Expression Plasmid
Highlights:
- Bacterial expression plasmid encodes a bacterial codon-optimized fragment of the SARS-CoV-2 nucleocapsid protein
- Plasmid was sequence verified
- Bacterial expression protocol can produce monomeric nucleocapsid protein (as confirmed by mass spectroscopy) with greater than 95% purify
- Purified nucleocapsid protein has been tested by collaborators for antigenicity in mice immunized against the recombinant protein as well as bats infected with SARS-CoV-2
- Protein expressed and purified from this plasmid is antigenic
- Protein can be used for ELISAs as well as other assays to indicate presence of SARS-CoV-2 nucleocapsid protein antibodies in patient samples
Anti-SARS-CoV-2 Nucleocapsid Protein [mBG17] Expression Plasmids
Highlights:
- Recognizes SARS-CoV2 Nucleocapsid protein
- mBG17 did show cross reaction with SARS-CoV nucleocapsid protein and minor cross-reaction with HuCoV-NL63 nucleocapsid protein was observed by ELISA,
- no cross-reaction was observed with other coronavirus N proteins.
- Suitable for ELISA, immunofluorescence assay, and western blot applications
Anti-SARS-CoV-2 Nucleocapsid Protein [mBG86] Expression Plasmids
Highlights:
- Recognizes SARS-CoV2 Nucleocapsid protein
- mBG86 showed no cross reaction with SARS-CoV nucleocapsid or any of the other coronavirus N proteins tested by ELISA
- mBG86 is very specific for SARS-CoV-2 nucleocapsid protein.
- Suitable for ELISA, immunofluorescence assay, and western blot applications
Benefits/Applications
- Plasmid can be licensed for in-house production – allows for larger amounts of antigen for diagnostic testing
- SARS-CoV-2 nucleoprotein is a major diagnostic antigen for detection of active infection and immunity
Last updated: April 2020
