At a Glance
Researchers at Colorado State University have developed and characterized a panel of monoclonal antibodies targeting the SARS-CoV-2 nucleocapsid protein. The clones were tested for activity in western blots, ELISAs, and immunofluorescence assays with SARS-CoV-2 infected cells and for cross reactivity against SARS-CoV, HuCoV-NL63, and HuCoV-229E N protein. Overall, data provides a strong foundation for the use of the monoclonal antibodies to study SARS-CoV-2 N protein and development of novel diagnostic assays to detection of COVID-19.
Coronavirus nucleocapsid protein plays a key role in coating the viral RNA genome during virion formation, and overall pathogenesis. Additionally, the nucleocapsid protein has been used as a diagnostic marker for active coronavirus infection. There are very few antibody reagents currently available that are specific for SARS-CoV-2 nucleocapsid, as most monoclonal antibodies that are commercially available were generated against SARS-CoV nucleocapsid protein and are cross-reactive for SARS-CoV-2 nucleocapsid. There is a significant need for new antibodies to be produced with increased specificity and utility for detecting SARS-CoV-2 nucleocapsid protein for both the research community and the necessity to develop therapeutics.
- Clones were screened by ELISA, Western Blot, and Immunofluorescence assays
- Cross-reactivity of antibodies was tested against SARS-CoV, HuCoV-NL63, and HuCoV-229E N protein
- Validated high specificity towards SARS-CoV-2 nucleocapsid protein across several diagnostic and research assays
- Antibodies specifically target SARS-CoV-2 nucleocapsid
- Nucleocapsid protein is a diagnostic marker for active coronavirus infection
- Application in ELISA assays, reducing/denatured western blots, immunofluorescence assays, sandwich ELISAs, gene sequences, and others